Catalog Number - 59150-01

MicroBee C18™ is a traditional octadecylsilane (C18) reversed-phase HPLC stationary phase that has been trusted by laboratories for many years. As part of the Cogent™ Similars product line, it provides a cost-effective alternative for established analytical methods while delivering the hydrophobic retention characteristics expected from a classic C18 chemistry. The end-capped, Type-A silica support is bonded with a straight-chain C18 ligand, making it particularly useful for laboratories operating legacy methods and compendial procedures.

59150-01 is a 150 mm x 3.9 mm ID analytical column packed with 10 µm, 125Å MicroBee C18 particles. This dimension closely matches the format used in many historical HPLC methods developed on Microbondapak™ and similar columns, making it especially useful for method transfer and replacement applications. The 150 mm length provides a balance of resolution and analysis time, while the 3.9 mm ID format typically performs well at flow rates of approximately 0.8–1.5 mL/min on conventional HPLC systems.

This USP L1 column is well suited for hydrophobic and neutral compounds analyzed under reversed-phase conditions. The traditional C18 chemistry provides reliable retention for a broad range of analytes and is particularly effective for long-chain fatty acids, pharmaceutical compounds, and other lipophilic molecules. As one of the most popular MicroBee C18 configurations, it remains a preferred choice for laboratories seeking a dependable alternative to legacy C18 column methods.

Key Benefits

• Traditional end-capped C18 stationary phase

• Suitable alternative for USP L1 methods

• Compatible with many legacy Microbondapak™ methods

• Popular replacement column format

• Excellent for hydrophobic and neutral compounds

• Effective for long-chain fatty acid analysis

• 125Å pore size for broad analyte compatibility

• Cost-effective Cogent Similars column

Typical Applications

• USP L1 methods

• Reversed-phase HPLC

• Method transfer projects

• Pharmaceutical analysis

• Quality control testing

• Long-chain fatty acid analysis

• Lipophilic compound separations

• Routine analytical HPLC

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee C18

• USP Classification: L1

• Particle Size: 10 µm

• Particle Type: Type-A Silica

• Bonded Phase: C18 (Octadecylsilane)

• Endcapped: Yes

• Pore Size: 125Å

• Column Dimensions: 3.9 mm ID x 150 mm

• Length: 150 mm

• Recommended Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Important Notes

• One of the most popular MicroBee C18 column configurations

• Designed for reversed-phase chromatography

• Traditional end-capped C18 chemistry

• Ideal for legacy method replacement

• Compatible with conventional HPLC systems

• Suitable for hydrophobic and neutral compounds

• Excellent alternative for many USP L1 methods

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